Coding

Part:BBa_K2306006:Design

Designed by: Guillermo Serena Ruiz   Group: iGEM17_TUDelft   (2017-10-04)


Cytosolic-abundant heat soluble protein 94205 (CAHS 94205)

This Biobrick features the gene sequence that encodes for the production of the tardigrade intrinsically disordered protein (TDP) "CAHS 94205". This is one of the several heat soluble proteins found in the tardigrades responsible for the ability of these microscopic animals to survive the harshest conditions. Upon desiccation, tardigrade proteins form a glass matrix capable of protecting other fragile biological components. Therefore, this tardigrade protein can be combined with other perishable biological material (e.g. proteins) to increase its stability against dehydration or even enable the possibility of stabilizing material with just a previous drying step.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 459
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 178
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

The sequence was already codon-optimized for E. Coli, and we made sure that there were no forbidden restriction sites present.

Source

The sequence of this part is based on that from the supplementary material of the paper by Boothby, T. C., et al. "Tardigrades Use Intrinsically Disordered Proteins to Survive Desiccation." Molecular Cell 65(6): 975-984.e975.

References

Boothby et al., 2017